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tslp  (R&D Systems)


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    Structured Review

    R&D Systems tslp
    Tslp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tslp/Recombinant+Mouse+TSLP+Protein/pm41986877-37-48-49
    Average 94 stars, based on 41 article reviews
    tslp - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: IL-33 promotes transcriptional and metabolic adaptations of tissue-resident Th2 cells
    Article Snippet: .. The cells were cultured with human IL-2 (PeproTech, 200-02-1mg; 100 Units/ml) alone or together with IL-33 (R&D Systems, no. 3626-ML-010/CF, 25 ng/ml) for 5 d. In certain experiments, alternative cytokines or cytokine combinations were used: IL-4 (PeproTech, 214-14; 10 ng/ml), IL-25 (R&D Systems 1399-IL-025/CF; 25 ng/ml), TSLP (R&D Systems, 555-TS-010/CF; 25 ng/ml). .. For CD3/CD28 stimulation, cells were cultured in a plate coated with an anti-CD3 antibody (5 μg/ml) in TCM supplemented with anti-CD28 (BioXCell, Clone 37.51; 2 μg/ml), IL-4 (PeproTech, 214-14; 10 ng/ml), IL-2 (PeproTech, 200-02-1mg; 100 Units/ml), and anti-IFN-γ (BioXCell, clone XMG1.2; 4 μg/ml).

    Article Title: IL-33 promotes transcriptional and metabolic adaptations of tissue-resident Th2 cells.
    Article Snippet: .. The cells were cultured with human IL-2 (PeproTech, 200-02-1mg; 100 Units/ml) alone or together with IL-33 (R&D Systems, no. 3626-ML-010/CF, 25 ng/ ml) for 5 d. In certain experiments, alternative cytokines or cytokine combinations were used: IL-4 (PeproTech, 214-14; 10 ng/ ml), IL-25 (R&D Systems 1399-IL-025/CF; 25 ng/ml), TSLP (R&D Systems, 555-TS-010/CF; 25 ng/ml). .. For CD3/CD28 stimulation, cells were cultured in a plate coated with an anti-CD3 antibody (5 μg/ml) in TCM supplemented with anti-CD28 (BioXCell, Clone 37.51; 2 μg/ml), IL-4 (PeproTech, 214-14; 10 ng/ml), IL-2 (PeproTech, 200-02-1mg; 100 Units/ml), and anti-IFN-γ (BioXCell, clone XMG1.2; 4 μg/ml).

    Modification:

    Article Title: Relevance of Thymic Stromal Lymphopoietin on the Pathogenesis of Glioblastoma: Role of the Neutrophil
    Article Snippet: .. Reagents and antibodies were purchased as follows: Ficoll-Hypaque from GE Healthcare Bio-Sciences AB (Uppsala, Sweden), RPMI-1640 culture media and Fetal bovine serum (FBS) from Invitrogen (Carlsbad, CA, USA), Dulbecco’s Modified Eagle’s Medium (DMEM; 12,800,017) from Thermo Fisher (Carlsbad, CA, USA), PE conjugated mouse antibody directed to CD11b and isotype controls from BD Bioscience (San Jose, CA, USA), TSLP from R&D Systems (Minneapolis, MN, USA), TSLPR antibody from Biolegend (San Diego, CA, USA), Programed death-ligand 1 (PDL1) and REGF antibodies from Biolegend (San Diego, CA, USA), and TMZ Sigma (St. Louis, MO, USA). ..

    Immunofluorescence:

    Article Title: Comparative analyses of Netherton syndrome patients and Spink5 conditional knock-out mice uncover disease-relevant pathways.
    Article Snippet: Antibodies to the following antigens were used for flow cytometry: CD3-FITC (BD Biosciences, #555274), CD4-APC/Cy7 (BD Pharmingen, #552051), CD8a-PE (Sony Biotechnology, #1103540), CD44-Pacific Blue (Biolegend, #103020), CD25-PerCP/Cy5.5 (Sony Biotechnology, #1109560), TCRβ-PE/Cy7 (Biolegend, #109222), TCR Vδ4-BV605 (BD Biosciences, #745116), IgM-APC (Sony Biotechnology, #2632545), CD45R/B220-PerpCP/Cy5.5 (Sony Biotechnology, #1116180). .. Primary antibodies used for immunofluorescence staining of antigens on paraffin sections: LEKTI (Cloud Clone Corp., #PAH145Mu01), S100A8 (Cell Signaling Technology, #47310), S100A9 (Cell Signaling Technology, #73425), KRT6A (Biolegend, #905701), LOR (Biolegend, #PRB-145P), TSLP (R&D Systems, #AF555), IL-36A (R&D Systems, #AF2297), IVL (Biolegend, #924401), CDH1 (Cell Signaling Technology, #3195), KRT10 (Biolegend, #905401 and #905403), KRT14 (Abcam, #ab181595), COMMUNICATIONS BIOLOGY | (2024) 7:152 | https://doi.org/10.1038/s42003-024-05780-y | www.nature.com/commsbio 19 KRT14 (Biolegend, #906004), KRT5 (Biolegend, #905903), Ki67 (Abcam, #ab15580), IL-24 (R&D Systems, #MAB2786), pSTAT3(Tyr705) (Cell Signaling Technology, #9145), FOXP3 (Cell Signaling Technology, #12653), IL-17A (Abcam, #ab79056), CD19 (Cell Signaling Technology, #90176), CD3 (Abcam, #ab11089), CD34 (Abcam, # ab8158), VIMENTIN (Cell Signaling Technology, #5741), KLK5 (Abbexa, #abx100977), KLK6 (Abbexa, # abx100979), KLK7 (R&D Systems, # AF2624), KLK13 (Abbexa, # abx129912) and KLK14 (Abcam, # ab278500). .. Primary antibodies used for immunofluorescence staining of antigens on cryosections: CD4 (BD Pharmingen, #553727), CD3e (BD Pharmingen, #555273), CD8a (BD Pharmingen, #553027), CXCL3 (ThermoFisherScientific, #PA5-103136), F4/80 (Abcam, #ab6640), DSP (Proteintech, #25318-1-AP), FLG (Biolegend, #PRB-417), DSG1 (Santa Cruz Biotechnology, #sc20114), and Ly6G/C (Abcam, NIMP-R14, #ab2557).

    Article Title: Comparative analyses of Netherton syndrome patients and Spink5 conditional knock-out mice uncover disease-relevant pathways
    Article Snippet: Antibodies to the following antigens were used for flow cytometry: CD3-FITC (BD Biosciences, #555274), CD4-APC/Cy7 (BD Pharmingen, #552051), CD8a-PE (Sony Biotechnology, #1103540), CD44-Pacific Blue (Biolegend, #103020), CD25-PerCP/Cy5.5 (Sony Biotechnology, #1109560), TCRβ-PE/Cy7 (Biolegend, #109222), TCR Vδ4-BV605 (BD Biosciences, #745116), IgM-APC (Sony Biotechnology, #2632545), CD45R/B220-PerpCP/Cy5.5 (Sony Biotechnology, #1116180). .. Primary antibodies used for immunofluorescence staining of antigens on paraffin sections: LEKTI (Cloud Clone Corp., #PAH145Mu01), S100A8 (Cell Signaling Technology, #47310), S100A9 (Cell Signaling Technology, #73425), KRT6A (Biolegend, #905701), LOR (Biolegend, #PRB-145P), TSLP (R&D Systems, #AF555), IL-36A (R&D Systems, #AF2297), IVL (Biolegend, #924401), CDH1 (Cell Signaling Technology, #3195), KRT10 (Biolegend, #905401 and #905403), KRT14 (Abcam, #ab181595), KRT14 (Biolegend, #906004), KRT5 (Biolegend, #905903), Ki67 (Abcam, #ab15580), IL-24 (R&D Systems, #MAB2786), pSTAT3(Tyr705) (Cell Signaling Technology, #9145), FOXP3 (Cell Signaling Technology, #12653), IL-17A (Abcam, #ab79056), CD19 (Cell Signaling Technology, #90176), CD3 (Abcam, #ab11089), CD34 (Abcam, # ab8158), VIMENTIN (Cell Signaling Technology, #5741), KLK5 (Abbexa, #abx100977), KLK6 (Abbexa, # abx100979), KLK7 (R&D Systems, # AF2624), KLK13 (Abbexa, # abx129912) and KLK14 (Abcam, # ab278500). .. Primary antibodies used for immunofluorescence staining of antigens on cryosections: CD4 (BD Pharmingen, #553727), CD3e (BD Pharmingen, #555273), CD8a (BD Pharmingen, #553027), CXCL3 (ThermoFisherScientific, #PA5-103136), F4/80 (Abcam, #ab6640), DSP (Proteintech, #25318-1-AP), FLG (Biolegend, #PRB-417), DSG1 (Santa Cruz Biotechnology, #sc20114), and Ly-6G/C (Abcam, NIMP-R14, #ab2557).

    Staining:

    Article Title: Comparative analyses of Netherton syndrome patients and Spink5 conditional knock-out mice uncover disease-relevant pathways.
    Article Snippet: Antibodies to the following antigens were used for flow cytometry: CD3-FITC (BD Biosciences, #555274), CD4-APC/Cy7 (BD Pharmingen, #552051), CD8a-PE (Sony Biotechnology, #1103540), CD44-Pacific Blue (Biolegend, #103020), CD25-PerCP/Cy5.5 (Sony Biotechnology, #1109560), TCRβ-PE/Cy7 (Biolegend, #109222), TCR Vδ4-BV605 (BD Biosciences, #745116), IgM-APC (Sony Biotechnology, #2632545), CD45R/B220-PerpCP/Cy5.5 (Sony Biotechnology, #1116180). .. Primary antibodies used for immunofluorescence staining of antigens on paraffin sections: LEKTI (Cloud Clone Corp., #PAH145Mu01), S100A8 (Cell Signaling Technology, #47310), S100A9 (Cell Signaling Technology, #73425), KRT6A (Biolegend, #905701), LOR (Biolegend, #PRB-145P), TSLP (R&D Systems, #AF555), IL-36A (R&D Systems, #AF2297), IVL (Biolegend, #924401), CDH1 (Cell Signaling Technology, #3195), KRT10 (Biolegend, #905401 and #905403), KRT14 (Abcam, #ab181595), COMMUNICATIONS BIOLOGY | (2024) 7:152 | https://doi.org/10.1038/s42003-024-05780-y | www.nature.com/commsbio 19 KRT14 (Biolegend, #906004), KRT5 (Biolegend, #905903), Ki67 (Abcam, #ab15580), IL-24 (R&D Systems, #MAB2786), pSTAT3(Tyr705) (Cell Signaling Technology, #9145), FOXP3 (Cell Signaling Technology, #12653), IL-17A (Abcam, #ab79056), CD19 (Cell Signaling Technology, #90176), CD3 (Abcam, #ab11089), CD34 (Abcam, # ab8158), VIMENTIN (Cell Signaling Technology, #5741), KLK5 (Abbexa, #abx100977), KLK6 (Abbexa, # abx100979), KLK7 (R&D Systems, # AF2624), KLK13 (Abbexa, # abx129912) and KLK14 (Abcam, # ab278500). .. Primary antibodies used for immunofluorescence staining of antigens on cryosections: CD4 (BD Pharmingen, #553727), CD3e (BD Pharmingen, #555273), CD8a (BD Pharmingen, #553027), CXCL3 (ThermoFisherScientific, #PA5-103136), F4/80 (Abcam, #ab6640), DSP (Proteintech, #25318-1-AP), FLG (Biolegend, #PRB-417), DSG1 (Santa Cruz Biotechnology, #sc20114), and Ly6G/C (Abcam, NIMP-R14, #ab2557).

    Article Title: Comparative analyses of Netherton syndrome patients and Spink5 conditional knock-out mice uncover disease-relevant pathways
    Article Snippet: Antibodies to the following antigens were used for flow cytometry: CD3-FITC (BD Biosciences, #555274), CD4-APC/Cy7 (BD Pharmingen, #552051), CD8a-PE (Sony Biotechnology, #1103540), CD44-Pacific Blue (Biolegend, #103020), CD25-PerCP/Cy5.5 (Sony Biotechnology, #1109560), TCRβ-PE/Cy7 (Biolegend, #109222), TCR Vδ4-BV605 (BD Biosciences, #745116), IgM-APC (Sony Biotechnology, #2632545), CD45R/B220-PerpCP/Cy5.5 (Sony Biotechnology, #1116180). .. Primary antibodies used for immunofluorescence staining of antigens on paraffin sections: LEKTI (Cloud Clone Corp., #PAH145Mu01), S100A8 (Cell Signaling Technology, #47310), S100A9 (Cell Signaling Technology, #73425), KRT6A (Biolegend, #905701), LOR (Biolegend, #PRB-145P), TSLP (R&D Systems, #AF555), IL-36A (R&D Systems, #AF2297), IVL (Biolegend, #924401), CDH1 (Cell Signaling Technology, #3195), KRT10 (Biolegend, #905401 and #905403), KRT14 (Abcam, #ab181595), KRT14 (Biolegend, #906004), KRT5 (Biolegend, #905903), Ki67 (Abcam, #ab15580), IL-24 (R&D Systems, #MAB2786), pSTAT3(Tyr705) (Cell Signaling Technology, #9145), FOXP3 (Cell Signaling Technology, #12653), IL-17A (Abcam, #ab79056), CD19 (Cell Signaling Technology, #90176), CD3 (Abcam, #ab11089), CD34 (Abcam, # ab8158), VIMENTIN (Cell Signaling Technology, #5741), KLK5 (Abbexa, #abx100977), KLK6 (Abbexa, # abx100979), KLK7 (R&D Systems, # AF2624), KLK13 (Abbexa, # abx129912) and KLK14 (Abcam, # ab278500). .. Primary antibodies used for immunofluorescence staining of antigens on cryosections: CD4 (BD Pharmingen, #553727), CD3e (BD Pharmingen, #555273), CD8a (BD Pharmingen, #553027), CXCL3 (ThermoFisherScientific, #PA5-103136), F4/80 (Abcam, #ab6640), DSP (Proteintech, #25318-1-AP), FLG (Biolegend, #PRB-417), DSG1 (Santa Cruz Biotechnology, #sc20114), and Ly-6G/C (Abcam, NIMP-R14, #ab2557).

    Isolation:

    Article Title: Inhaled LTI-03 for Idiopathic Pulmonary Fibrosis: A Randomized Dose Escalation Study
    Article Snippet: .. Protein isolation and preparation of DBB samples for ELISAs were performed as previously described ( ) according to manufacturer’s instructions for COL1A1 (#ab210966, abcam), CXCL7 (#LS-F4967-1, LS Bio), GAL7 (#DY1339, R&D Systems), IL-11 (#DY218, R&D Systems), TSLP (#DY1398, R&D Systems). ..

    Multiplex Assay:

    Article Title: Simultaneous quantitative analysis of multiple atopic dermatitis biomarkers using ag nanorods on Micro post arrays
    Article Snippet: Multiple biomarkers associated with atopic dermatitis were simultaneously quantified using Ag nanorods on micro post arrays (Ag NMPA).. The Ag NMPA substrate consists of 500 nm-high metallic nanorods formed on top of 50 μm-high microstructures.. This was fabricated utilizing UV micro-imprinting and glancing angle deposition (GLAD).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Simultaneous quantitative analysis of multiple atopic dermatitis biomarkers using ag nanorods on Micro post arrays
    Article Snippet: Multiple biomarkers associated with atopic dermatitis were simultaneously quantified using Ag nanorods on micro post arrays (Ag NMPA).. The Ag NMPA substrate consists of 500 nm-high metallic nanorods formed on top of 50 μm-high microstructures.. This was fabricated utilizing UV micro-imprinting and glancing angle deposition (GLAD).

    Article Title: Indeno[1,2,3-cd]pyrene enhances the sensitivity of airway epithelial cells to ferroptosis and aggravates asthma.
    Article Snippet: • PM2.5 and Indeno[1,2,3-cd]pyrene (IP) promoted ferroptosis aggravating



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    Image Search Results


    The Expression and origin of TSLP in AAA. (A) Serum TSLP concentrations patients with AAA (n=632) compared with healthy controls (n=24,036) in UKB. (B) The schematic diagram is presented below for reference. 10-12-week- aged male mice received a PPE induction at day 0, with aortic sampling performed at day 14. (C) The comparison of TSLP mRNA expression in the aortas from the sham and AAA groups at day 14 (n=6 mice per group). (D) Temporal expression profile of TSLP mRNA in the murine aorta at indicated time points after AAA induction, compared to the baseline level (n=6 mice per group). (E-G) Representative immunofluorescence images of murine AAA sections stained for TSLP (red), cell-specific markers (green), and DAPI (blue). (E) Co-staining with CD45 (leukocyte marker). (F) Co-staining with vimentin (fibroblast marker). (G) Co-staining with α-SMA (smooth muscle cell marker) (n=4 mice per group). Data are presented as mean ± SEM. Scale bar: 50 μm. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (the Mann–Whitney U test was used in A, unpaired two-tailed Student’s t-test was used in C, and one-way ANOVA was used in D).

    Journal: Frontiers in Immunology

    Article Title: Thymic stromal lymphopoietin promotes abdominal aortic aneurysm formation by regulating macrophage polarization

    doi: 10.3389/fimmu.2026.1767913

    Figure Lengend Snippet: The Expression and origin of TSLP in AAA. (A) Serum TSLP concentrations patients with AAA (n=632) compared with healthy controls (n=24,036) in UKB. (B) The schematic diagram is presented below for reference. 10-12-week- aged male mice received a PPE induction at day 0, with aortic sampling performed at day 14. (C) The comparison of TSLP mRNA expression in the aortas from the sham and AAA groups at day 14 (n=6 mice per group). (D) Temporal expression profile of TSLP mRNA in the murine aorta at indicated time points after AAA induction, compared to the baseline level (n=6 mice per group). (E-G) Representative immunofluorescence images of murine AAA sections stained for TSLP (red), cell-specific markers (green), and DAPI (blue). (E) Co-staining with CD45 (leukocyte marker). (F) Co-staining with vimentin (fibroblast marker). (G) Co-staining with α-SMA (smooth muscle cell marker) (n=4 mice per group). Data are presented as mean ± SEM. Scale bar: 50 μm. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (the Mann–Whitney U test was used in A, unpaired two-tailed Student’s t-test was used in C, and one-way ANOVA was used in D).

    Article Snippet: Exogenous TSLP treatment: Recombinant mouse TSLP protein (HY- P70626 , MCE, USA) was administered via intraperitoneal injection starting on day 1 after PPE induction at a dose of 20 mg/kg, and then every other day thereafter.

    Techniques: Expressing, Sampling, Comparison, Immunofluorescence, Staining, Marker, MANN-WHITNEY, Two Tailed Test

    Genetic ablation of TSLP receptor attenuates experiment al AAA severity. (A) Schematic of the experimental design utilizing wildtype (WT) and Tslpr − / − mice in the PPE-induced AAA model. (B) Quantification of the maximum abdominal aortic diameter in WT and Tslpr − / − mice at day 14 post-PPE induction (n=5 mice per group). (C) Representative images of elastic Elastica Van Gieson (EVG) staining of aortic tissue. (D) Elastin degradation scores evaluated by EVG staining (n=5 mice per group). (E–J) Representative immunohistochemical staining and quantitative analysis of CD68 (E, F) , MMP2 (G, H) and MMP9 (I, J) expression in the aortic wall. Positive areas are presented as a percentage of the total area (n=5 mice per group). Scale bars: (A) 1mm; (C) 100 μm (top), 50 μm (bottom); (E, G, I) 500 μm (top), 40 μm (bottom). All quantitative data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test). Red arrows indicate representative positive staining areas.

    Journal: Frontiers in Immunology

    Article Title: Thymic stromal lymphopoietin promotes abdominal aortic aneurysm formation by regulating macrophage polarization

    doi: 10.3389/fimmu.2026.1767913

    Figure Lengend Snippet: Genetic ablation of TSLP receptor attenuates experiment al AAA severity. (A) Schematic of the experimental design utilizing wildtype (WT) and Tslpr − / − mice in the PPE-induced AAA model. (B) Quantification of the maximum abdominal aortic diameter in WT and Tslpr − / − mice at day 14 post-PPE induction (n=5 mice per group). (C) Representative images of elastic Elastica Van Gieson (EVG) staining of aortic tissue. (D) Elastin degradation scores evaluated by EVG staining (n=5 mice per group). (E–J) Representative immunohistochemical staining and quantitative analysis of CD68 (E, F) , MMP2 (G, H) and MMP9 (I, J) expression in the aortic wall. Positive areas are presented as a percentage of the total area (n=5 mice per group). Scale bars: (A) 1mm; (C) 100 μm (top), 50 μm (bottom); (E, G, I) 500 μm (top), 40 μm (bottom). All quantitative data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test). Red arrows indicate representative positive staining areas.

    Article Snippet: Exogenous TSLP treatment: Recombinant mouse TSLP protein (HY- P70626 , MCE, USA) was administered via intraperitoneal injection starting on day 1 after PPE induction at a dose of 20 mg/kg, and then every other day thereafter.

    Techniques: Staining, Immunohistochemical staining, Expressing, Two Tailed Test

    Therapeutic neutralization of TSLP protects against AAA development. (A) Schematic diagram of the experimental protocol for anti-mouse TSLP antibody (anti-TSLP) administration in the PPE-induced AAA model. (B) Comparison of the maximum abdominal aortic diameter in normal saline-treated and TSLP-treated mice at day 14 (n=6 mice per group). (C, D) Representative histological staining and corresponding quantification for Elastica Van Gieson (EVG) (n=6 mice per group). (E–J) Representative images and quantitative analysis of immunohistochemical staining for CD68 (E, F) , MMP2 (G, H) and MMP9 (I, J) expression in the aortic wall. Positive areas are presented as a percentage of the total area (n=6 mice per group). Scale bars: (A) 1mm; (C) 500 μm; (E, G, I) 200 μm (top), 50 μm (bottom). All quantitative data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test). Ctrl, control. Red arrows indicate representative positive staining areas.

    Journal: Frontiers in Immunology

    Article Title: Thymic stromal lymphopoietin promotes abdominal aortic aneurysm formation by regulating macrophage polarization

    doi: 10.3389/fimmu.2026.1767913

    Figure Lengend Snippet: Therapeutic neutralization of TSLP protects against AAA development. (A) Schematic diagram of the experimental protocol for anti-mouse TSLP antibody (anti-TSLP) administration in the PPE-induced AAA model. (B) Comparison of the maximum abdominal aortic diameter in normal saline-treated and TSLP-treated mice at day 14 (n=6 mice per group). (C, D) Representative histological staining and corresponding quantification for Elastica Van Gieson (EVG) (n=6 mice per group). (E–J) Representative images and quantitative analysis of immunohistochemical staining for CD68 (E, F) , MMP2 (G, H) and MMP9 (I, J) expression in the aortic wall. Positive areas are presented as a percentage of the total area (n=6 mice per group). Scale bars: (A) 1mm; (C) 500 μm; (E, G, I) 200 μm (top), 50 μm (bottom). All quantitative data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test). Ctrl, control. Red arrows indicate representative positive staining areas.

    Article Snippet: Exogenous TSLP treatment: Recombinant mouse TSLP protein (HY- P70626 , MCE, USA) was administered via intraperitoneal injection starting on day 1 after PPE induction at a dose of 20 mg/kg, and then every other day thereafter.

    Techniques: Neutralization, Comparison, Saline, Staining, Immunohistochemical staining, Expressing, Two Tailed Test, Control

    Exogenous TSLP administration aggravates AAA in mice. (A) Schematic diagram of the experimental protocol for recombinant TSLP administration in the PPE-induced AAA model. (B) Comparison of the maximum abdominal aortic diameter in normal saline-treated and TSLP-treated mice at day 14 (n=6 mice per group). (C, D) Representative histological staining and corresponding quantification for EVG (n=6 mice per group). (E–J) Representative images and quantitative analysis of immunohistochemical staining for CD68 (E, F) , MMP2 (G, H) and MMP9 (I, J) expression in the aortic wall. Positive areas are presented as a percentage of the total area (n=6 mice per group). Scale bars: (A) 1mm; (C) 500 μm; (E, G, I) 200 μm (top), 40 μm (bottom). All quantitative data presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, no significant difference (unpaired two-tailed Student’s t-test). Ctrl, control. Red arrows indicate representative positive staining areas.

    Journal: Frontiers in Immunology

    Article Title: Thymic stromal lymphopoietin promotes abdominal aortic aneurysm formation by regulating macrophage polarization

    doi: 10.3389/fimmu.2026.1767913

    Figure Lengend Snippet: Exogenous TSLP administration aggravates AAA in mice. (A) Schematic diagram of the experimental protocol for recombinant TSLP administration in the PPE-induced AAA model. (B) Comparison of the maximum abdominal aortic diameter in normal saline-treated and TSLP-treated mice at day 14 (n=6 mice per group). (C, D) Representative histological staining and corresponding quantification for EVG (n=6 mice per group). (E–J) Representative images and quantitative analysis of immunohistochemical staining for CD68 (E, F) , MMP2 (G, H) and MMP9 (I, J) expression in the aortic wall. Positive areas are presented as a percentage of the total area (n=6 mice per group). Scale bars: (A) 1mm; (C) 500 μm; (E, G, I) 200 μm (top), 40 μm (bottom). All quantitative data presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, no significant difference (unpaired two-tailed Student’s t-test). Ctrl, control. Red arrows indicate representative positive staining areas.

    Article Snippet: Exogenous TSLP treatment: Recombinant mouse TSLP protein (HY- P70626 , MCE, USA) was administered via intraperitoneal injection starting on day 1 after PPE induction at a dose of 20 mg/kg, and then every other day thereafter.

    Techniques: Recombinant, Comparison, Saline, Staining, Immunohistochemical staining, Expressing, Two Tailed Test, Control

    TSLP signaling regulates the immune microenvironment and macrophage polarization in AAA. (A, B) viSNE plots visualizing the high-dimensional CyTOF data, depicting the overall immune cell composition and the specific distribution of TSLP. (C) Quantitative analysis of the total macrophage frequency among CD11b + immune cells in the aortic tissues. (D, E) The ratio of M1 to M2 macrophages in the aortic tissues of WT and Tslpr − / − mice. (F) Gating strategy for M1 (CD86 + ) and M2 (CD206 + ) macrophages in RAW264.7 cells and BMDMs by flow cytometry. (G, H) Flow cytometry analysis and quantification of the percentages of M1 (CD86 + ) and M2 (CD206 + ) macrophages in RAW264.7 cells (G) and BMDMs (H) following polarization and TSLP treatment. (I) Proportions of M1 and M2 macrophages in PMA-differentiated THP-1 cells treated with or without TSLP. Cell stimulation conditions: M1 polarization control (Ctrl), 100ng/ml LPS + 20ng/ml IFN-γ for 24h; M1 polarization with TSLP (TSLP), 100ng/ml LPS + 20ng/ml IFN-γ+20ng/ml TSLP for 24h; M2 polarization control (Ctrl), 20ng/ml IL-4 for 24h; M2 polarization with TSLP (TSLP), 20ng/ml IL-4 + 20ng/ml TSLP for 24h (n=4–6 per group). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test). M1, M1 macrophages; M2, M2 macrophages; DCs, dendritic cells; NK cells, natural killer cells.

    Journal: Frontiers in Immunology

    Article Title: Thymic stromal lymphopoietin promotes abdominal aortic aneurysm formation by regulating macrophage polarization

    doi: 10.3389/fimmu.2026.1767913

    Figure Lengend Snippet: TSLP signaling regulates the immune microenvironment and macrophage polarization in AAA. (A, B) viSNE plots visualizing the high-dimensional CyTOF data, depicting the overall immune cell composition and the specific distribution of TSLP. (C) Quantitative analysis of the total macrophage frequency among CD11b + immune cells in the aortic tissues. (D, E) The ratio of M1 to M2 macrophages in the aortic tissues of WT and Tslpr − / − mice. (F) Gating strategy for M1 (CD86 + ) and M2 (CD206 + ) macrophages in RAW264.7 cells and BMDMs by flow cytometry. (G, H) Flow cytometry analysis and quantification of the percentages of M1 (CD86 + ) and M2 (CD206 + ) macrophages in RAW264.7 cells (G) and BMDMs (H) following polarization and TSLP treatment. (I) Proportions of M1 and M2 macrophages in PMA-differentiated THP-1 cells treated with or without TSLP. Cell stimulation conditions: M1 polarization control (Ctrl), 100ng/ml LPS + 20ng/ml IFN-γ for 24h; M1 polarization with TSLP (TSLP), 100ng/ml LPS + 20ng/ml IFN-γ+20ng/ml TSLP for 24h; M2 polarization control (Ctrl), 20ng/ml IL-4 for 24h; M2 polarization with TSLP (TSLP), 20ng/ml IL-4 + 20ng/ml TSLP for 24h (n=4–6 per group). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test). M1, M1 macrophages; M2, M2 macrophages; DCs, dendritic cells; NK cells, natural killer cells.

    Article Snippet: Exogenous TSLP treatment: Recombinant mouse TSLP protein (HY- P70626 , MCE, USA) was administered via intraperitoneal injection starting on day 1 after PPE induction at a dose of 20 mg/kg, and then every other day thereafter.

    Techniques: Flow Cytometry, Cell Stimulation, Control, Two Tailed Test

    Transcriptomic profiling reveals TSLP-mediated activation of pro-inflammatory pathways in macrophages. RAW 264.7 was stimulated with 100ng/ml LPS + 20ng/ml IFN-γ+20ng/ml TSLP (TSLP group) or 100ng/ml LPS + 20ng/ml IFN-γ for 24h (Control group) and then subjected to RNA-sequencing. (A) Principal component analysis (PCA). (B) Clustering diagram of differentially expressed gene (DEG) pattern. (C) Volcano plot displays DEGs. (D, E) Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of downregulated genes. Data are from n=4 samples per group.

    Journal: Frontiers in Immunology

    Article Title: Thymic stromal lymphopoietin promotes abdominal aortic aneurysm formation by regulating macrophage polarization

    doi: 10.3389/fimmu.2026.1767913

    Figure Lengend Snippet: Transcriptomic profiling reveals TSLP-mediated activation of pro-inflammatory pathways in macrophages. RAW 264.7 was stimulated with 100ng/ml LPS + 20ng/ml IFN-γ+20ng/ml TSLP (TSLP group) or 100ng/ml LPS + 20ng/ml IFN-γ for 24h (Control group) and then subjected to RNA-sequencing. (A) Principal component analysis (PCA). (B) Clustering diagram of differentially expressed gene (DEG) pattern. (C) Volcano plot displays DEGs. (D, E) Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of downregulated genes. Data are from n=4 samples per group.

    Article Snippet: Exogenous TSLP treatment: Recombinant mouse TSLP protein (HY- P70626 , MCE, USA) was administered via intraperitoneal injection starting on day 1 after PPE induction at a dose of 20 mg/kg, and then every other day thereafter.

    Techniques: Activation Assay, Control, RNA Sequencing

    Therapeutic neutralization of TSLP protects against AAA development. (A) Schematic diagram of the experimental protocol for anti-mouse TSLP antibody (anti-TSLP) administration in the PPE-induced AAA model. (B) Comparison of the maximum abdominal aortic diameter in normal saline-treated and TSLP-treated mice at day 14 (n=6 mice per group). (C, D) Representative histological staining and corresponding quantification for Elastica Van Gieson (EVG) (n=6 mice per group). (E–J) Representative images and quantitative analysis of immunohistochemical staining for CD68 (E, F) , MMP2 (G, H) and MMP9 (I, J) expression in the aortic wall. Positive areas are presented as a percentage of the total area (n=6 mice per group). Scale bars: (A) 1mm; (C) 500 μm; (E, G, I) 200 μm (top), 50 μm (bottom). All quantitative data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test). Ctrl, control. Red arrows indicate representative positive staining areas.

    Journal: Frontiers in Immunology

    Article Title: Thymic stromal lymphopoietin promotes abdominal aortic aneurysm formation by regulating macrophage polarization

    doi: 10.3389/fimmu.2026.1767913

    Figure Lengend Snippet: Therapeutic neutralization of TSLP protects against AAA development. (A) Schematic diagram of the experimental protocol for anti-mouse TSLP antibody (anti-TSLP) administration in the PPE-induced AAA model. (B) Comparison of the maximum abdominal aortic diameter in normal saline-treated and TSLP-treated mice at day 14 (n=6 mice per group). (C, D) Representative histological staining and corresponding quantification for Elastica Van Gieson (EVG) (n=6 mice per group). (E–J) Representative images and quantitative analysis of immunohistochemical staining for CD68 (E, F) , MMP2 (G, H) and MMP9 (I, J) expression in the aortic wall. Positive areas are presented as a percentage of the total area (n=6 mice per group). Scale bars: (A) 1mm; (C) 500 μm; (E, G, I) 200 μm (top), 50 μm (bottom). All quantitative data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test). Ctrl, control. Red arrows indicate representative positive staining areas.

    Article Snippet: Anti-TSLP antibody treatment: Anti-TSLP antibody (HY-P990150, MCE, USA) was administered via intraperitoneal injection starting on day 1 after PPE induction at a dose of 20 mg/kg, and then every other day thereafter.

    Techniques: Neutralization, Comparison, Saline, Staining, Immunohistochemical staining, Expressing, Two Tailed Test, Control